Mouse SEMA6A/Semaphorin-6A ELISA Kit PicoKine®, Carrier-free

• Background:

  Semaphorin-6A is a protein that in humans is encoded by the SEMA6A gene. This gene is mapped to 18; 18 C. Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal.

• :

  heparin, EDTA or citrate

• Description:

  Mouse SEMA6A ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Sema6a in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	101	94	96	98	97	2.58	2.6%
  Sample 2	465	451	513	472	475	23.06	4.8%
  Sample 3	1684	1888	1678	1813	1765	88.82	5.0%

  *number of samples for each test n=16.

• Synonyms:

  Semaphorin-6A; Semaphorin Q; Sema Q; Semaphorin VIA; Sema VIA; Semaphorin-6A-1; SEMA6A-1

• Gene Name:

  sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A

• Gene ID:

  20358

• UniProt:

  O35464

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: G19-T649

• Tissue Specificity:

  Particularly high levels in spinal cord, cerebellum, metencephalon, superior and inferior colliculus, diencephalon, olfactory bulb and eye.

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

• Detection Range:

  62.5 pg/ml - 4,000 pg/ml

• Sensitivity:

  <50 pg/ml

• Function:

  Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Specificity:

  Natural and recombinant mouse SEMA6A

• Subcellular Location:

  Cell membrane. Single-pass type I membrane protein.