Mouse SEMA3F/Semaphorin-3F ELISA Kit PicoKine®, Carrier-free

• Background:

  Semaphorin-3F is a protein that in humans is encoded by the SEMA3F gene. It is mapped to 9; 9 F1. The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin 3 family, consisting of human semaphorins 3A-G, chicken collapsin, and mouse semaphorins 3A-G, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K , a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family.

• :

  heparin, EDTA or citrate

• Description:

  Mouse SEMA3F ELISA Kit PicoKine® (96 Tests). Quantitate Mouse SEMA3F in cell culture supernatants, serum and plasma (heparin, EDTA or citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	59	70	64	60	63	4.32	6.8%
  Sample 2	231	224	225	261	235	15.10	6.4%
  Sample 3	896	856	928	913	898	26.89	2.9%

  *number of samples for each test n=16.

• Synonyms:

  Semaphorin-3F; Semaphorin IV; Sema IV; Sema3f

• Gene Name:

  sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F

• Gene ID:

  20350

• UniProt:

  O88632

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: A19-T785

• Tissue Specificity:

  Expressed ubiquitously in adulthood. During embryogenesis, expressed in subregions of the central nervous system and various other tissues like skin, kidney, lung and intestine.

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA or citrate)

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Specificity:

  Natural and recombinant mouse SEMA3F

• Subcellular Location:

  Secreted.