Human TNFRSF18/GITR ELISA Kit PicoKine®, Carrier-free

• Background:

  Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

• :

  heparin or EDTA

• Description:

  Human TNFRSF18/GITR ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	135	136	127	144	135	6.02	4.4%
  Sample 2	705	808	735	671	729	50.53	6.9%
  Sample 3	1313	1243	1354	1475	1346	84.26	6.2%

  *number of samples for each test n=16.

• Synonyms:

  Tumor necrosis factor receptor superfamily member 18;Activation-inducible TNFR family receptor;Glucocorticoid-induced TNFR-related protein;CD357;TNFRSF18;AITR, GITR;UNQ319/PRO364;

• Gene Name:

  Tumor necrosis factor receptor superfamily member 18

• Gene ID:

  8784

• UniProt:

  Q9Y5U5

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: Q26-E161

• Tissue Specificity:

  Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  62.5 pg/ml - 4,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  26000 MW

• Specificity:

  Natural and recombinant human TNFRSF18

• Subcellular Location:

  Isoform 1: Cell membrane; Single-pass type I membrane protein.

• Protein Name:

  Tumor necrosis factor receptor superfamily member 18