Human TNFRSF18/GITR ELISA Kit PicoKine®, Carrier-free

Human TNFRSF18/GITR ELISA Kit PicoKine®, Carrier-free

• Background: Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
• Description: Human TNFRSF18/GITR ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Tumor necrosis factor receptor superfamily member 18;Activation-inducible TNFR family receptor;Glucocorticoid-induced TNFR-related protein;CD357;TNFRSF18;AITR, GITR;UNQ319/PRO364;
• Gene Name: Tumor necrosis factor receptor superfamily member 18
• Gene ID: 8784
• UniProt: Q9Y5U5
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q26-E161
• Tissue Specificity: Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 62.5 pg/ml - 4,000 pg/ml
• Sensitivity: <10 pg/ml
• Function: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant human TNFRSF18
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	135	136	127	144	135	6.02	4.4%
  Sample 2	705	808	735	671	729	50.53	6.9%
  Sample 3	1313	1243	1354	1475	1346	84.26	6.2%

  *number of samples for each test n=16.
• : heparin or EDTA
• Calculated Molecular Weight: 26000 MW
• Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein.
• Protein Name: Tumor necrosis factor receptor superfamily member 18