Mouse NGF/NGF Beta ELISA Kit PicoKine®, Carrier-free

• Background:

  Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.

• Description:

  Mouse NGF/NGF Beta ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ngf in cell culture supernatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	99	87	83	82	87	6.75	7.7%
  Sample 2	235	231	230	203	224	12.69	5.6%
  Sample 3	910	887	898	936	907	18.22	2%

  *number of samples for each test n=16.

• Synonyms:

  Beta-nerve growth factor;Beta-NGF;Ngf;Ngfb;

• Gene Name:

  Beta-nerve growth factor

• Gene ID:

  18049

• UniProt:

  P01139

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: S122-G241

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants and serum.

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <1 pg/ml

• Function:

  Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI. .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  27077 MW

• Specificity:

  Natural and recombinant mouse NGF

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the NGF-beta family.

• Protein Name:

  Beta-nerve growth factor