Human MCP-1 / CCL2 ELISA Kit PicoKine®, Carrier-free

Human MCP-1 / CCL2 ELISA Kit PicoKine®, Carrier-free

• Background: Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.
• Description: Human MCP-1 / CCL2 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL2 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: C-C motif chemokine 2;HC11;Monocyte chemoattractant protein 1;Monocyte chemotactic and activating factor;MCAF;Monocyte chemotactic protein 1;MCP-1;Monocyte secretory protein JE;Small-inducible cytokine A2;CCL2;MCP1, SCYA2;
• Gene Name: C-C motif chemokine 2
• Gene ID: 6347
• UniProt: P13500
• Reactivity: Human
• Cross Reactivity: There is cross-reactivity with human Eotaxin, MCP-3<1%.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: Q24-T99
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Sensitivity: <1 pg/ml
• Function: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant human MCP-1
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	31	33	37	35	34	2.23	6.5%
  Sample 2	159	139	156	158	153	8.15	5.3%
  Sample 3	477	421	423	456	444	23.46	5.2%

  *number of samples for each test n=16.
• : heparin, EDTA or citrate
• Calculated Molecular Weight: 11025 MW
• Subcellular Location: Secreted.
• Sequence Similarities: Belongs to the intercrine beta (chemokine CC) family.
• Protein Name: C-C motif chemokine 2