Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit, Carrier-free

Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit, Carrier-free

• Background: Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
• Description: Human A2M/Alpha2-Macroglobulin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human A2M in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Gene Name: alpha-2-macroglobulin
• Gene ID: 2
• UniProt: P01023
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Target protein isolated from human plasma
• Applications: ELISA
• Standard: 40 ng/tube
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 625 pg/ml - 40,000 pg/ml
• Sensitivity: <300 pg/ml
• Components: Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
• Storage Conditions: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
• Specificity: Natural and recombinant Human A2M
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	1336	1254	1452	1396	1360	61.18	4.5%
  Sample 2	3774	3674	3364	3354	3542	254.99	7.2%
  Sample 3	26412	33012	29451	29456	29583	1804.55	6.1%

  *number of samples for each test n=16.
• : heparin or EDTA
• Protein Name: Alpha-2-macroglobulin