Human CXCL10/IP-10 PicoKine® Quick ELISA Kit, Carrier-free

• Background:

  Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

• :

  heparin or EDTA

• Description:

  Human CXCL10/IP-10 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CXCL10 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	66	55	61	58	60	3.54	5.9%
  Sample 2	245	263	269	254	258	7.22	2.8%
  Sample 3	894	935	842	971	911	49.17	5.4%

  *number of samples for each test n=16.

• Gene Name:

  chemokine (C-X-C motif) ligand 10

• Gene ID:

  3627

• UniProt:

  P02778

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: V22-P98

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <1 pg/ml

• Components:

  Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

• Storage Conditions:

  Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

• Specificity:

  Natural and recombinant Human CXCL10

• Protein Name:

  C-X-C motif chemokine 10