Anti-Apc5/ANAPC5 Antibody Picoband®, PE Conjugate

• Background:

  Anaphase-promoting complex subunit 5 is an enzyme that in humans is encoded by the ANAPC5 gene. This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

• Description:

  Boster Bio Anti-Apc5/ANAPC5 Antibody Picoband® catalog # A08280-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Neuropeptides B/W receptor type 1; G-protein coupled receptor 7; NPBWR1; GPR7

• Gene Name:

  neuropeptides B and W receptor 1

• Gene ID:

  51433

• UniProt:

  Q9UJX4

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human Apc5/ANAPC5 recombinant protein (Position: M1-D266).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Found in cerebellum and frontal cortex. Detected at high levels in hippocampus, amygdala and trachea; at moderate levels in fetal brain, pituitary gland and prostate. Not in caudate, accumbens, kidney or liver. Also detected at high levels in lung carcinoma.

• Applications:

  WB,ICC,IF,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals. Has a higher affinity for neuropeptide B.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

• References & Citations:

  1. Jorgensen, P. M., Graslund, S., Betz, R., Stahl, S., Larsson, C., Hoog, C. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene 262: 51-59, 2001. 2. Turnell, A. S., Stewart, G. S., Grand, R. J. A., Rookes, S. M., Martin, A., Yamano, H., Elledge, S. J., Gallimore, P. H. The APC/C and CBP/p300 cooperate to regulate transcription and cell-cycle progression. Nature 438: 690-695, 2005. 3. Yu, H., Peters, J.-M., King, R. W., Page, A. M., Hieter, P., Kirschner, M. W. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science 279: 1219-1222, 1998.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  85 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Cell membrane. Multi-pass membrane protein.