Anti-Cyclin A2/CCNA2 Antibody Picoband®, PE Conjugate

Anti-Cyclin A2/CCNA2 Antibody Picoband®, PE Conjugate

• Background: Cyclin A2, known as CCNA2, is mapped to 4q27. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. And Cyclin A2 is synthesized at the onset of S phase and localizes to the nucleus, where the cyclin A2-CDK2 complex is implicated in the initiation and progression of DNA synthesis. Phosphorylation of CDC6 and MCM4 by the cyclin A2-CDK2 complex prevents re-replication of DNA during the cell cycle.
• Description: Boster Bio Anti-Cyclin A2/CCNA2 Antibody Picoband® catalog # PB9424. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Cyclin-A2;Cyclin-A;CCNA2;CCN1, CCNA;
• Gene Name: Cyclin-A2
• Gene ID: 890
• UniProt: P20248
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E.coli-derived human Cyclin A2 recombinant protein (Position: A10-K168). Human Cyclin A2 shares 74.5% amino acid (aa) sequence identity with mouse Cyclin A2.
• Clonality: Polyclonal
• Tissue Specificity: Ubiquitous.
• Applications: Flow Cytometry,IF,IHC,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
• Components: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• References & Citations: 1. Gong D, Ferrell JE (Sep 2010). "The roles of cyclin A2, B1, and B2 in early and late mitotic events". Mol. Biol. Cell 21(18): 3149–61. 2. Yam CH, Fung TK, Poon RY (Aug 2002). "Cyclin A in cell cycle control and cancer". Cell. Mol. Life Sci. 59 (8): 1317–26.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 48551 MW
• Observed Molecular Weight: 55 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
• Subcellular Location: Nucleus . Cytoplasm . Cytoplasmic when associated with SCAPER.
• Sequence Similarities: Belongs to the cyclin family. Cyclin AB subfamily.
• Protein Name: Cyclin-A2