Anti-CD8 alpha/Cd8a Antibody Picoband®

• Background:

  The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

• Description:

  Boster Bio Anti-CD8 alpha/Cd8a Antibody Picoband® catalog # A02236-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD8a; Cd8a; Lyt-2; Lyt2

• Gene Name:

  CD8 antigen, alpha chain

• UniProt:

  P01731

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196) .

• Clonality:

  Polyclonal

• Applications:

  ELISA, Flow Cytometry, IHC, WB

• Field of Research:

  Adaptive Immunity, Immunology, T Cells

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  6

• Other Gene Names:

  Cd8a

• Subcellular Location:

  Membrane; Single-pass type I membrane protein.