Anti-Transcription factor Spi-B SPIB Antibody

• Background:

  SPIB is a member of the ETS transcription factor family that influences lymphoid development and activity and binds the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the ETS domain (1) . SPIB is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC) (2,3) . It promotes the development of plasmacytoid dendritic cells (pDCs) or natural interferon (IFN) -producing cells. SPIB may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation (3,4) .

• Description:

  Boster Bio Anti-Transcription factor Spi-B SPIB Antibody (Catalog # A02805-1) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Spi-B transcription factor, SPI-B

• Gene Name:

  SPIB

• UniProt:

  Q01892

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Cross Reactivity:

  SPIB antibody is human, mouse and rat reactive. At least four isoforms of SPIB are known to exist; this antibody will detect all but isoform 3.

• Immunogen:

  SPIB antibody was raised against a 17 amino acid peptide near the carboxy terminus of human SPIB. The immunogen is located within amino acids 200 - 250 of SPIB.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.

• Applications:

  ELISA, WB, IHC-P, IF

• Field of Research:

  Stem Cells, Immunology

• Purification:

  SPIB antibody is affinity chromatography purified via peptide column.

• Concentration:

  1 mg/mL

• Form:

  Liquid

• Function:

  Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN) -producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.

• Molecular Weight:

  28819 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  SPIB antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

• Fragment:

  IgG

• Other Gene Names:

  Spi-B transcription factor (Spi-1/PU.1 related)

• Subcellular Location:

  Isoform 1: Nucleus

• Prediction Reactivity:

  Pig

• Protein Name:

  Spi-B transcription factor (Spi-1/PU.1 related)