Anti-AP-2 complex subunit mu AP2M1 Antibody

• Background:

  The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA) -4 protein. At least two isoforms of AP2M1 are known to exist.

• Description:

  Boster Bio Anti-AP-2 complex subunit mu AP2M1 Antibody (Catalog # A06179-1) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Mu2, AP50, CLAPM1, KIAA0109, AP-2 complex subunit mu, AP-2 mu chain

• Gene Name:

  AP2M1

• UniProt:

  Q96CW1

• Host:

  Chicken

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  AP2M1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human AP2M1. The immunogen is located within amino acids 360 - 410 of AP2M1.

• Clonality:

  Polyclonal

• Applications:

  ELISA, WB, IHC-P, IF

• Field of Research:

  Homeostasis

• Purification:

  AP2M1 Antibody is affinity chromatography purified via peptide column.

• Concentration:

  1 mg/mL

• Form:

  Liquid

• Function:

  Component of the adaptor protein complex 2 (AP-2) . Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X- X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane- associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity) . Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity) .

• Molecular Weight:

  49655 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  AP2M1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgY

• Other Gene Names:

  Adaptor-related protein complex 2, mu 1 subunit

• Subcellular Location:

  Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. .

• Prediction Reactivity:

  Bovine, Chicken

• Protein Name:

  Adaptor-related protein complex 2, mu 1 subunit