Anti-Phospho-Nucleophosmin (T199) Rabbit Monoclonal Antibody

Anti-Phospho-Nucleophosmin (T199) Rabbit Monoclonal Antibody

• Description: Boster Bio Anti-Phospho-Nucleophosmin (T199) Rabbit Monoclonal Antibody catalog # P00450. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
• Synonyms: RAF proto-oncogene serine/threonine-protein kinase;2.7.11.1;Proto-oncogene c-RAF;cRaf;Raf-1;RAF1;RAF;
• CAS Number: 9007-83-4
• Gene Name: RAF proto-oncogene serine/threonine-protein kinase
• UniProt: P06748
• Host: Rabbit
• Reactivity: Human
• Immunogen: A synthesized peptide derived from human Phospho-Nucleophosmin (T199)
• Clonality: Monoclonal
• Clone: Clone: 28N18
• Applications: WB,IHC,ICC,IF,Flow Cytometry
• Purification: Affinity-chromatography
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form: Liquid
• Function: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2- antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. .
• Storage Conditions: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 73052 MW
• Observed Molecular Weight: 38-40 kDa
• Fragment: IgG
• Applications Notes: WB 1:500-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  FC 1:50
• Phospho Site: T199