Anti-NPM (phospho-T199) NPM1 Antibody

• Description:

  Boster Bio Anti-NPM (phospho-T199) NPM1 Antibody catalog # A00450T199-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Nucleophosmin; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM1; NPM;

• Gene Name:

  NPM1

• UniProt:

  P06748

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human NPM around the phosphorylation site of Threonine 199.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification:

  The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Form:

  Liquid

• Function:

  Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486) . In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029) .

• Molecular Weight:

  32575 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Other Gene Names:

  Nucleophosmin

• Subcellular Location:

  Nucleus, nucleolus

• Protein Name:

  Nucleophosmin