Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit

Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit

• Background: Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high absorbance lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
• Description: Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human APOA1/Apolipoprotein A-I in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• CAS Number: 7732-18-5
• Gene Name: APOA1
• Gene ID: 335
• UniProt: P02647
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: D25-Q267
• Applications: ELISA
• Detection Range: 3.12 ng/ml - 200 ng/ml
• : heparin or EDTA
• Sample Type: cell culture supernatants, serum, plasma (heparin, EDTA) and urine
• Sensitivity: <50 pg/ml
• Storage Conditions: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
• Reproducibility:

  Reproducibility

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	8188	7998	8004	7332	7880	330.96	4.2%
  Sample 2	19829	19750	20658	19659	19974	619.19	3.1%
  Sample 3	93784	86245	87457	88574	89015	4005.67	4.5%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant Human APOA1