X-Gluc (cyclohexanamine)

• Description:

  X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator) . X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue) [1].

• UNSPSC:

  12171500

• Hazard Statement:

  H302, H315, H319, H335

• Target:

  Fluorescent Dye; β-glucuronidase

• Type:

  Dye Reagents

• Related Pathways:

  Metabolic Enzyme/Protease; Others

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/x-gluc-cyclohexanamine.html

• Concentration:

  10mM

• Purity:

  99.96

• Solubility:

  DMSO : 100 mg/mL (ultrasonic)

• Smiles:

  NC1CCCCC1.O[C@H]([C@@H](O)[C@@H]2O)[C@@H](O[C@@H]2C(O)=O)OC3=CNC4=C3C(Cl)=C(Br)C=C4

• Molecular Formula:

  C20H26BrClN2O7

• Molecular Weight:

  521.79

• Precautions:

  H302, H315, H319, H335

• References & Citations:

  [1]Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51 (5) :402-404.

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  4°C (Powder, protect from light)

• Scientific Category:

  Dye Reagents

• Clinical Information:

  No Development Reported

• CAS Number:

  [114162-64-0]