Recombinant Cynomolgus IL-4 Receptor Subunit Alpha/IL-4RA (C-Fc)

• Background:

  Interleukin-4 receptor subunit alpha (IL-4RA), alos known as Soluble IL-4 receptor subunit alpha, belongs to the type I cytokine receptor family and type 4 subfamily. It expressed in both Th1 and Th2 cells. It functions as receptor for both interleukin 4 and interleukin 13 and couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and chemokine and mucus production at sites of allergic inflammation. In certain cell types, IL-4RA can signal through activation of insulin receptor substrates, IRS1/IRS2. The functional IL4 receptor is formed by initial binding of IL4 to IL4R. Subsequently it recruits to the complex of the common gamma chain. In immune cells, IL-4RA creates a type I receptor. In non-immune cells, it forms a type II receptor with of IL13RA1. IL4R can also interact with the IL13/IL13RA1 complex to form a similar type II receptor and interacts with the SH2-containing phosphatases, PTPN6/SHIP1, PTPN11/SHIP2 and INPP5D/SHIP.

• Abbreviation:

  IL-4RA

• Synonyms:

  CD124; IL-4R-alpha; IL-4RA; IL4-BP; Interleukin-4 receptor subunit alpha; Soluble IL-4R-alpha

• UniProt:

  G7Q0S7

• Accession Number:

  G7Q0S7

• Expression System:

  HEK293 Cells

• Tag:

  C-Fc

• Sequence:

  Met26-Arg232

• Applications:

  Cell culture

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells.The ED₅₀ for this effect is 6.02 ng/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  50.7 kDa

• Observed Molecular Weight:

  65 - 90 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM Tris-HCl, 100mM Glycine, pH 7.5.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Cynomolgus macaques