Anti-GUS | Beta-glucuronidase

• Background:

  GUS (Beta-glucuronidase) is an enzyme which when incubated with colorless or non-fluorescent substrates, can catalyze reaction of their transformation into coloured or fluorescent products.

• HS Code:

  38221900

• Gene ID:

  946149

• UniProt:

  P05804

• Host:

  Rabbit

• Reactivity:

  GUS

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  KLH-conjugated synthetic peptide derived from Escherichia coli GUS protein at amino acid 358.

• Clonality:

  Polyclonal

• Applications:

  Immunolocalization (IL), Western blot (WB)

• Field of Research:

  GalB/GUS/LUC

• Dilution:

  1 : 10 000 (WB)

• Purity:

  Serum

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 50 µl of sterile water

• Molecular Weight:

  Depends upon a MW of a protein which is expressed with GUS

• Additionnal Information:

  This antibody will not work with pCAMBIA vectors due to mutation starting at amino acid position 358

• References & Citations:

  Mastsushima and Matsuo (2023) . Removal of plant endogenous proteins from tobacco leaf extract by freeze–thaw treatment for purification of recombinant proteins. Plant Sci. 2023 Dec 10:339:111953.doi: 10.1016/j.plantsci.2023.111953. Xiumei et al. (2022) Pathogenesis-related protein 1 suppresses oomycete pathogen by targeting against AMPK kinase complex, Journal of Advanced Research, 2022, ISSN 2090-1232, https://doi.org/10.1016/j.jare.2022.02.002.Xiao et al. (2022) An amino acid transporter-like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper. Plant Biotechnol J. 2022 Jun 9. doi: 10.1111/pbi.13869. Epub ahead of print. PMID: 35678495. (Immunolocalization) Nizkorodova et al. (2020) . The Effect of Translation Promoting Site (TPS) on Protein Expression in E. coli Cells. Mol Biotechnol (2020) . doi.org/10.1007/s12033-020-00251-1Nikorodova and Isakov (2019) . New insights into the mechanism of action of e-element enhancer in E.coli. Eurasian Journal of Applied Biotechnology 2/2019

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4