Recombinant Mouse Siglec-F (C-6His)

Recombinant Mouse Siglec-F (C-6His)

• Background: Siglec 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system. This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Mouse Siglec-F cDNA encodes a 569 amino acid polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, three Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. The expression of Siglec-F is restricted to the cells of myelomonocytic lineage. Mouse Siglec-F is likely an ortholog of human Siglec-5. Unlike many human CD33-related Siglecs, which show similar binding to both alpha 2,3- and alpha 2,6-linked sialic acids, mouse Siglec-F preferentially recognize alpha 2,3-linked sialic acid.
• Abbreviation: Siglec-F
• Synonyms: SAF2, SIGLEC-8, SIGLEC8L, Siglec-F, SiglecF, sialic acid binding Ig like lectin 8
• CAS Number: 9000-83-3
• UniProt: Q920G3
• Accession Number: Q920G3
• Host: HEK293 Cells
• Origin Species: Mouse
• Tag: C-His
• Sequence: Asp18-Thr437
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 55-70 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.