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Recombinant Human Siglec-8 (C-mFc)

CAT: 0763-PKSH033960-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0763-PKSH033960-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Siglec-8 is also known as SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L and sialic acid binding Ig like lectin 8, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Siglec-8 is expressed on eosinophils, basophils, and mast cells, and it shows a binding preference for the carbohydrate 6-O sulfated sLex. At the tissue level, Siglec-8 mRNA was found to be most highly expressed in lung, PBMCs, spleen, and kidney. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 115 aa cytoplasmic domain with two tyrosine based signaling motifs. Alternative splicing generates additional isoforms that either lack most of the second Ig-like domain or have a substituted cytoplasmic domain without the signaling motifs. Cross-linking of Siglec-8 inhibits Fc epsilon RI alpha induced mast cell degranulation. It also induces eosinophil apoptosis, an effect which is enhanced by the eosinophil-activating cytokines IL-5, IL-33, and GM-CSF.
Abbreviation
Siglec-8
Synonyms
CD329 antigen; CDw329; SAF-2; SAF2; SAF2SAF-2; Siglec-8; Siglec8
UniProt
Q9NYZ4
Accession Number
Q9NYZ4
Expression System
HEK293 Cells
Tag
C-mFc
Sequence
Met17-Ala363
Field of Research
Signal Transduction; immunology
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
64.2 kDa
Observed Molecular Weight
80-100 kDa
Species
Human

UniProtKB · Q9NYZ4

Sialic acid-binding Ig-like lectin 8

SIGL8_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9NYZ4
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SIGLEC8
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Siglec-8
EC number
—
Processing
Precursor
Secondary accessions
Q7Z728
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesion

PTM

Disulfide bondGlycoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

Ligand

Lectin

Cellular component

Membrane
Product Datasheet
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