Recombinant Mouse Nectin-4 (C-Fc)

• Background:

  Nectin-4 (PVRL4) isatypeItransmembraneglycoproteinwhichbelongstothenectinfamilyofIgsuperfamilyproteins.ItcontainstwoIg-likeC2-typedomainsandoneIg-likeV-typedomain.PVRL4seemstobeinvolvedincelladhesionthroughtrans-homophilicand-heterophilicinteractions, thelatterincludingspecificallyinteractionswithnectin-1.Itdoesnotactasreceptorforalpha-herpesvirusentryintocells.Itispredominantlyexpressedinplacenta, theembryoandbreastcarcinoma.Butitisnotdetectedinnormalbreastepithelium.Thesolubleformisproducedbyproteolyticcleavageatthecellsurface (shedding), probablybyADAM17.Mutationsinthisgenearethecauseofectodermaldysplasia-syndactylysyndrometype 1, an autosomal recessive disorder.

• Abbreviation:

  Nectin-4

• Synonyms:

  Ig superfamily receptor LNIR; LNIR; Nectin-4; PRR4; PVRL4; Poliovirus receptor-related protein 4

• UniProt:

  Q8R007

• Accession Number:

  Q8R007

• Expression System:

  HEK293 Cells

• Tag:

  C-Fc

• Sequence:

  Gly31-Ser349

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  61.4 kDa

• Observed Molecular Weight:

  75 - 85 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse