Recombinant Human CD112/Nectin-2 Protein

• Background:

  Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

• Synonyms:

  CD112; HVEB; Herpes Virus Entry Mediator B; Herpesvirus Entry Mediator B; HveB; Nectin-2; PRR2; PVRL2; Poliovirus Receptor-Related Protein 2

• UniProt:

  Q92692

• Accession Number:

  NP_002847.1

• Expression System:

  HEK293 Cells

• Tag:

  None

• Sequence:

  Met 1-Leu 360

• Applications:

  ELISA

• Field of Research:

  Microbiology; immunology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Immobilized human CD112 at 10 μg/ml (100 μl/well) can bind human DNAM1-Fch with a linear range of 1. 25-10 μg/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  36.2 kDa

• Observed Molecular Weight:

  48 kDa

• Formulation:

  Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human