Recombinant Mouse TIMP1 Protein (His Tag)

• Background:

  Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity) . Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.

• Abbreviation:

  TIMP1

• Synonyms:

  Collagenase inhibitor 16C8 fibroblast; EPA; Erythroid-potentiating activity; Metalloproteinase inhibitor 1; TIMP-1; TPA-S1; TPA-induced protein; Timp; Timp-1; Tissue inhibitor of metalloproteinases 1

• UniProt:

  P12032

• Accession Number:

  P12032-1

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Pro28-Arg205

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 80% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  19.9 kDa

• Observed Molecular Weight:

  22.18 kDa

• Species:

  Mouse