Recombinant Mouse CD27/TNFRSF7 Protein (His & Fc Tag)

Recombinant Mouse CD27/TNFRSF7 Protein (His & Fc Tag)

• Background: CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.
• Synonyms: CD27 antigen, CD27L receptor, S152, T-cell activation antigen CD27, Tnfrsf7, Tp55, Tumor necrosis factor receptor superfamily member 7
• CAS Number: 9000-83-3
• UniProt: P41272
• Accession Number: NP_001028298.1
• Host: HEK293 Cells
• Origin Species: Mouse
• Tag: C-His-Fc
• Sequence: Met 1-Arg 182
• Applications: ELISA
• Purity: > 94 % as determined by reducing SDS-PAGE.
• Bioactivity: Immobilized recombinant mouse CD27 at 2 μg/ml (100 μl/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 60-65 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.