Recombinant Mouse TIGIT Protein (Fc Tag)

Recombinant Mouse TIGIT Protein (Fc Tag)

• Background: TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40. In addition, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.
• Synonyms: T-cell immunoreceptor with Ig and ITIM domains, TIGIT, V-set and transmembrane domain-containing protein 3, VSTM3
• CAS Number: 9000-83-3
• UniProt: PP86176
• Accession Number: PP86176
• Host: HEK293 Cells
• Origin Species: Mouse
• Tag: C-hFc
• Sequence: Met1-Gly141
• Applications: ELISA
• Purity: > 90 % as determined by reducing SDS-PAGE.
• Bioactivity: Immobilized mouse PVR-His at 10 μg/ml (100 μl/well) can bind mouse TIGIT-Fc, The EC50 of mouse TIGIT-Fc is 0.25-0.55 μg/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 52 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.