Recombinant Mouse TIGIT Protein (His Tag)
CAT:
763-PKSM040426
Size:
100 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Recombinant Mouse TIGIT Protein (His Tag)
- Background: TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40. In addition, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.
- Synonyms: T-cell immunoreceptor with Ig and ITIM domains, TIGIT, V-set and transmembrane domain-containing protein 3, VSTM3
- CAS Number: 9000-83-3
- Accession Number: NP_001139797.1
- Host: HEK293 Cells
- Origin Species: Mouse
- Tag: C-His
- Sequence: Met 1-Gly 141
- Applications: ELISA
- Purity: > 90 % as determined by reducing SDS-PAGE.
- Bioactivity: Immobilized mouse TIGIT-His at 10 μg/ml (100 μl/well) can bind mouse PVR-Fch, The EC50 of mouse PVR-Fch is 0.31-0.73 μg/ml.
- Reconstitution: Please refer to the printed manual for detailed information.
- Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
- Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.