Recombinant Human TIGIT Protein (mFc Tag)

• Background:

  T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the CD28 family within the Ig superfamily of proteins. TIGIT is expressed on NK cells and subsets of activated; memory and regulatory T cells; and particularly on follicular helper T cells within secondary lymphoid organs. It binds to CD155 and Nectin-2 that appear on dendritic cells (DC) and endothelium. Ligation of TIGIT on T cells down-regulates TCR-mediated activation and subsequent proliferation; while NK cell TIGIT ligation blocks NK cell cytotoxicity. Through CD155 and Nectin-2; which also interact with DNAM-1/CD226 and CD96/Tactile; TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other. In particular; TIGIT binding to CD155 can antagonize the effects of DNAM1.

• Abbreviation:

  TIGIT

• Synonyms:

  T-cell immunoreceptor with Ig and ITIM domains; TIGIT; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain-containing protein 3; VSIG9; VSTM3

• UniProt:

  Q495A1

• Accession Number:

  Q495A1

• Expression System:

  HEK293 Cells

• Tag:

  C-mFc

• Sequence:

  Met22-Pro141

• Field of Research:

  Immunology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  39.7 kDa

• Observed Molecular Weight:

  40-50 kDa

• Species:

  Human