Recombinant Mouse BACE2/Beta secretase 2 Protein (His Tag)

• Background:

  BACE2, also known as beta secretase 2, belongs to the peptidase A1 family. It is a protease known to be an important enzyme involved in the cellular pathways. BACE2 has been shown to interact with GGA1 and GGA2. It is the major β -secretase in vivo. BACE2 is located on chromosome 21 and may play a role in alzheimer's disease pathogenesis in down syndrome (DS) . Overexpression of BACE2 by lentivirus markedly reduced amyloid β protein production in primary neurons. Despite an extra copy of the BACE2 gene in DS and the increase of its transcription, BACE2 protein levels are unchanged.

• Synonyms:

  1110059C24Rik; AEPLC; AI850424; ALP56; ARP1; ASP21; BAE2; CDA13; CEAP1; DRAP

• UniProt:

  Q9JL18

• Accession Number:

  Q9JL18

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Met1-Pro462

• Applications:

  Enzyme

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2. The specific activity is > 50 pmoles/min/μg.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  49.2 kDa

• Observed Molecular Weight:

  55 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse