Recombinant Human PVRIG (C-mFc)

• Background:

  Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family. The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells. PVRIG may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals.

• Abbreviation:

  PVRIG

• Synonyms:

  C7orf15; C7orf15MGC138295; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; transmembrane protein PVRIG

• UniProt:

  Q6DKI7

• Accession Number:

  Q6DKI7

• Expression System:

  HEK293 Cells

• Tag:

  C-Fc

• Sequence:

  Thr41-Asp171

• Applications:

  BLI

• Field of Research:

  Cell biology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Loaded Biotinylated Human Nectin-2-His on His Biosensor, can bind Human PVRIG with an affinity constant of 168.5n M as determined in BLI assay.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  40.6 kDa

• Observed Molecular Weight:

  45-50 kDa

• Species:

  Human