Recombinant Human CD3 epsilon/CD3E (C-mFC)

• Background:

  T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) . TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

• Abbreviation:

  CD3 epsilon, CD3E

• Synonyms:

  CD3E; CD3e; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; T-Cell Surface Glycoprotein CD3 Epsilon Chain; T3E

• UniProt:

  P07766

• Accession Number:

  P07766

• Expression System:

  HEK293 Cells

• Tag:

  C-mFC

• Sequence:

  Gln22-Thr48

• Applications:

  ELISA

• Field of Research:

  Immunology; Stem cells

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Immobilized Anti-Human/Monkey CD3E mAb at 2μg/ml (100 μl/well) can bind Human CD3E-mFc.The ED50 of Human CD3E-mFc is 28.99 ng/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  28.5 kDa

• Observed Molecular Weight:

  32 - 40 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human