Recombinant Human Siglec-10 (C-Fc)

Recombinant Human Siglec-10 (C-Fc)

• Background: Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.
• Abbreviation: Siglec-10
• Synonyms: MGC126774, PRO940, SIGLEC10, SLG2, SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7, Siglec-10, Siglec-like protein 2, Siglec10, sialic acid-binding Ig-like lectin 10, siglec-like gene 2
• CAS Number: 9000-83-3
• UniProt: Q96LC7
• Accession Number: Q96LC7
• Host: HEK293 Cells
• Origin Species: Human
• Tag: C-Fc
• Sequence: Met17-Thr546
• Applications: ELISA
• Field of Research: Tags & Cell Markers
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Immobilized Anti-Human Siglec10 mAb at 2μg/ml (100 μl/well) can bind Human Siglec-10-Fc. The ED50 of Human Siglec-10-Fc is 9.18 ng/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 110-120 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.