Recombinant Human TNF-alpha/TNFA Protein
CAT:
763-PKSH033490-01
Size:
50 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Recombinant Human TNF-alpha/TNFA Protein
- Background: TNFα is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation; differentiation; inflammation; viral replication; tumorigenesis; autoimmune diseases and in viral; bacterial; fungal; and parasitic infections. Besides inducing hemorrhagic necrosis of tumors; TNF was found to be involved in tumorigenesis; tumor metastasis; viral replication; septic shock; fever; inflammation; and autoimmune diseases including Crohn’s disease; and rheumatoid arthritis as well as graft-versus-host disease.
- Abbreviation: TNF-alpha;TNFA
- Synonyms: Cachectin, TNF, TNF-Alpha, TNF-a, TNFA, TNFSF2, Tumor Necrosis Factor, Tumor Necrosis Factor Ligand Superfamily Member 2
- CAS Number: 9000-83-3
- UniProt: P01375
- Accession Number: P01375
- Host: E. coli
- Origin Species: Human
- Tag: None
- Sequence: Val77-Leu233
- Applications: Cell culture
- Field of Research: Signal Transduction;Cardiovascular;Cancer;immunology;metabolism;
- Purity: > 95 % as determined by reducing SDS-PAGE.
- Bioactivity: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-50 pg/ml.
- Reconstitution: Please refer to the printed manual for detailed information.
- Molecular Weight: 16 kDa
- Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
- Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.