Recombinant Human S100A8&S100A9 Heterodimer Protein (His Tag)

• Background:

  S100A8 is a member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. Calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase.

• Abbreviation:

  S100A8&S100A9 Heterodimer

• Synonyms:

  S100A8/S100A9 Heterodimer

• UniProt:

  P05109; P06702

• Accession Number:

  P05109&P06702

• Expression System:

  E.coli

• Tag:

  C-His

• Sequence:

  Met 1-Glu93 (S100A8) &Thr 2-Pro114 (S100A9)

• Field of Research:

  Signal Transduction; Cell biology; Neuroscience; Cancer; epigenetics and nuclear signal; immunology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  11.7&13.2 kDa

• Observed Molecular Weight:

  13&15 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of 20mM HEPES, 10% Glycerol, 150mM NaCl, 2.5mM EDTA, pH 7.4.

• Species:

  Human