Recombinant Human SENP7 Protein

• Background:

  Sentrin-Specific Protease 7 (SENP7) acts as a SUMO-2/3-specific protease. SENP7 is likely to regulate the metabolism of poly-SUMO-2/3 rather than SUMO-1 conjugation in vivo. SENP7 has a restricted substrate specificity, and displaying paralogue-specific isopeptidase activity. The C-terminal catalytic domain of SENP7 depolymerized poly-SUMO-2 chains but does not have activity against poly-SUMO-1 chains. SENP7 also had isopeptidase activity against di-SUMO-2- and SUMO-2-modified RanGAP1 (Ran GTPase-activating protein 1) but had limited activity against SUMO-1-modified RanGAP1.

• Abbreviation:

  SENP7

• Synonyms:

  KIAA1707; SENP7; SSP2; SUMO-1-Specific Protease 2; SUSP2; Sentrin-Specific Protease 7; Sentrin/SUMO-Specific Protease SENP7

• UniProt:

  Q9BQF6

• Accession Number:

  Q9BQF6

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Met695-Ala864

• Field of Research:

  Cell biology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  19.8 kDa

• Observed Molecular Weight:

  22 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of 20mM HEPES, 5% Glycerol, pH 7.4.

• Species:

  Human