Recombinant Human Osteonectin/SPARC Protein (His Tag)

• Background:

  Secreted Protein Acidic and Rich in Cysteine (SPARC) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family. SPARC has 286 amino acids and contains an EF-hand in C-termina domain, a follistatin-like domain with Kazal-like sequences. There are two calcium binding sites, one binds 5 - 8 Ca2+ with a low affinity and other on an EF-hand loop that binds a Ca2+ ion with a high affinity. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC regulate cell growth through interactions with the extracellular matrix (ECM) and cytokines. SPARC bind to numerous proteins of the ECM, affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC also binds several types of collagen, albumin, thrombospondin, PDGF and cell membranes.

• Abbreviation:

  Osteonectin; SPARC

• Synonyms:

  BM-40; Basement-Membrane Protein 40; ON; Osteonectin; SPARC; Secreted Protein Acidic and Rich in Cysteine

• UniProt:

  P09486

• Accession Number:

  P09486

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Ala18-Ile303

• Field of Research:

  Signal Transduction; Cell biology; Cardiovascular; Cancer; Developmental Biology; Stem cells

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  33.7 kDa

• Observed Molecular Weight:

  36 kDa

• Species:

  Human