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Human ON (Osteonectin) ELISA Kit

CAT: 0763-E-EL-H1351-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-H1351-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
ON
UniProt
P09486
Cross Reactivity
This kit recognizes Human ON in samples. No significant cross-reactivity or interference between Human ON and analogues was observed
Target
SPARC
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cell Biology; Cardiovascular; Developmental biology; Signal transduction; Stem cells
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ON. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ON and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ON, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ON. You can calculate the concentration of Human ON in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.31-20 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.19 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep4.1234
Citation 02
https://www.sciencedirect.com/science/article/pii/S0009279721003124
Citation 03
https://www.sciencedirect.com/science/article/pii/S0928098721000701
Citation 04
https://www.sciencedirect.com/science/article/pii/S1773224722003252
Citation 05
https://www.sciencedirect.com/science/article/pii/S0196978123000360
Citation 06
https://www.sciencedirect.com/science/article/pii/S0024320520312558
Citation 07
https://www.sciencedirect.com/science/article/pii/S0024320520307438
Citation 08
https://www.sciencedirect.com/science/article/pii/S1359511319304180
Citation 09
https://www.sciencedirect.com/science/article/pii/S187881812030815X
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human ON concentrations in serum, plasma and other biological fluids.

UniProtKB · P09486

SPARC

SPRC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09486
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SPARC
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
D3DQH9, Q6IBK4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Basement membraneExtracellular matrixSecreted

Ligand

CalciumCopperMetal-binding

Disease

Disease variantOsteogenesis imperfecta

PTM

Disulfide bondGlycoprotein

Domain

Signal

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