Recombinant Human NOL3 Protein

• Background:

  Nucleolar protein 3 is encoded by NOL3 gene. Multiple transcript variants encoding different isoforms have been found for this gene. So far; Nucleolar protein 3 has show to have two Isoforms. Isoform 1 may be involved in RNA splicing. Isoform 2 functions as an apoptosis repressor that blocks multiple modes of cell death. It inhibits extrinsic apoptotic pathways through two different ways. Firstly; it by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner; limiting the amount of soluble CASP8 available for DISC-mediated activation. It has been shown to down-regulate the enzyme activities of caspase 2; caspase 8 and tumor protein p53.

• Abbreviation:

  NOL3

• Synonyms:

  Apoptosis Repressor With CARD; Muscle-Enriched Cytoplasmic Protein; Myp; Nop30; Nucleolar Protein 3; Nucleolar Protein of 30 kDa

• UniProt:

  O60936

• Accession Number:

  O60936

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Met 1-Ser208

• Field of Research:

  Cell biology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  22.6 kDa

• Observed Molecular Weight:

  29 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of 20mM PB, 150mm NaCl, 1mM DTT, 1mM EDTA, 10% Glycerol, pH7.0.

• Species:

  Human