Recombinant Human FGF-1/FGFa Protein

Recombinant Human FGF-1/FGFa Protein

• Background: FGF acidic; also known as ECGF; FGF-1and HBGF-1; is a non-glycosylated heparin binding growth factor that is expressed in the brain; kidney; retina; smooth muscle cells; bone matrix; osteoblasts; astrocytes and endothelial cells. It is a mitogenic peptide that is produced by multiple cell types and stimulates the proliferation of cells of mesodermal; ectodermal; and endodermal origin. Its association with heparan sulfate is a prerequisite for activation of FGF receptors. Internalized FGF acidic migrates to the nucleus where it is phosphorylated by nuclear PKC delta; exported to the cytosol; dephosphorylated; and degraded. Intracellular FGF acidic inhibits p53 activity and proapoptotic signaling.
• Abbreviation: FGF-1;FGFa
• Synonyms: Acidic Fibroblast Growth Factor, ECGFHeparin-Binding Growth Factor 1, Endothelial Cell Growth Factor, FGF-1, FGF1, FGFA, Fibroblast Growth Factor 1, HBGF-1, aFGF
• CAS Number: 9000-83-3
• UniProt: P05230
• Accession Number: P05230
• Host: E. coli
• Origin Species: Human
• Tag: C-His
• Sequence: Phe16-Asp155
• Applications: Cell culture
• Field of Research: Signal Transduction;Cardiovascular;Neuroscience;Cancer;
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Bioactivity: Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <0.3 ng/mL. The specific activity of recombinant human FGF-1 is > 1 x 10⁶ IU/mg.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 18 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.