Mouse CFB (Complement Factor B) ELISA Kit

CAT: 0763-E-EL-M0334-01Size: 24 TestsDry Ice: NoHazardous: No

CAT#:0763-E-EL-M0334-01Size:24 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Abbreviation

CFB

UniProt

P04186

Cross Reactivity

This kit recognizes Mouse CFB in samples. No significant cross-reactivity or interference between Mouse CFB and analogues was observed

Target

AHUS4; BF; BFD; CFAB; FB; FBI12; GBG; H2-Bf; PBF2; Properdin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CFB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CFB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CFB. You can calculate the concentration of Mouse CFB in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0147651318301477

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse CFB concentrations in serum, plasma and other biological fluids.

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Mouse CFB (Complement Factor B) ELISA Kit

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