Recombinant Human DDR2 Kinase/CD167b Protein (Fc Tag)

Recombinant Human DDR2 Kinase/CD167b Protein (Fc Tag)

• Background: Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.
• Synonyms: CD167, MIG20a, NTRKR3, TKT, TYRO10
• CAS Number: 9000-83-3
• UniProt: Q16832
• Accession Number: NP_001014796.1
• Host: HEK293 Cells
• Origin Species: Human
• Tag: C-hFc
• Sequence: Met 1-Arg 399
• Applications: ELISA
• Field of Research: Cardiovascular;Neuroscience
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Immobilized Rat tail Collagen I at 10 μg/ml can bind recombinant human DDR2-Fc Chimera with a linear range of 2. 5-80 ng/ml. Scatchard analysis showed the affinity constant (Kd) of recombinant human DDR2-Fc Chimera bound to rat tail collagen I was 6. 8 nM.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 87 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.