Recombinant Human DDR2 Kinase/CD167b Protein (aa 422-855, His & GST Tag)

• Background:

  Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.

• Synonyms:

  CD167; MIG20a; NTRKR3; TKT; TYRO10

• UniProt:

  Q16832

• Accession Number:

  Q16832

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-His-GST

• Sequence:

  Arg422-Glu855

• Applications:

  Enzyme

• Field of Research:

  Cardiovascular; Neuroscience

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  The specific activity was determined to be 8 nmol/min/mg using synthetic AXLtide peptide (CKKSRGDYMTMQIG) as substrate.

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  77.1 kDa

• Observed Molecular Weight:

  77 kDa

• Formulation:

  Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol

• Species:

  Human