Recombinant Human TWF1/Twinfilin-1 Protein

• Background:

  Twinfilin-1; also known as Protein A6; Protein tyrosine kinase 9; TWF1 and PTK9; is a cytoplasm protein which belongs to theactin-binding proteins ADF family and Twinfilin subfamily. Twinfilin-1 (TWF1 / PTK9) is a highly conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. In addition to the mammalian twinfilin-1; a second protein with approximately 65% sequence identity to twinfilin-1 exists in mouse and humans. TWF1 / PTK9 is expressed at high levels in the colon; testis; ovary; prostate and lung. It is expressed at lower levels in the brain; bladder and heart. It is not detected in liver. TWF1 / PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization; likely by sequestering G-actin. By capping the barbed ends of filaments; it also regulates motility. TWF1 / PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

• Synonyms:

  A6; MGC23788; MGC41876; PTK9

• UniProt:

  Q12792

• Accession Number:

  Q12792-4

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Met 1-Asp 252

• Field of Research:

  Signal Transduction

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 94 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  29 kDa

• Observed Molecular Weight:

  36 kDa

• Species:

  Human