Recombinant Human CALR/Calreticulin Protein (His Tag)

• Background:

  Calreticulin is a multifunctional protein. It acts as a main Ca (2+) -binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction) ; rendering it inactive. The Ca2+ is bound with low affinity; but high capacity; and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum; calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo; as well as retinoic acid-induced neuronal differentiation. Therefore; calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.

• Synonyms:

  CRT; HEL-S-99n; RO; SSA; cC1qR

• UniProt:

  P27797

• Accession Number:

  P27797

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Met 1-Ala413

• Field of Research:

  Signal Transduction; Tags & Cell Markers

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  47.4 kDa

• Observed Molecular Weight:

  58 kDa

• Species:

  Human