Human CRT (Calreticulin) ELISA Kit

• Abbreviation:

  CRT

• UniProt:

  P27797

• Cross Reactivity:

  This kit recognizes Human CRT in samples. No significant cross-reactivity or interference between Human CRT and analogues was observed

• Target:

  Calreticulin; calregulin; CRP55; CaBP3; calsequestrin-like protein; endoplasmic reticulum resident protein 60; ERp60; CALR; CRT; RO; SSA; cC1qR

• Type:

  Sandwich-ELISA

• Applications:

  ELISA

• Field of Research:

  Cancer; Signal transduction; Tags and Cell Markers

• Assay Principle:

  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CRT. You can calculate the concentration of Human CRT in the samples by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3 h 30 min

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  0.16-10 ng/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  0.1 ng/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Citation 01:

  https://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.2c00499

• Citation 02:

  https://link.springer.com/article/10.1007/s10528-022-10309-1

• Detection Instrument:

  Microplate reader

• Species:

  Human

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Human CRT concentrations in serum, plasma and other biological fluids.