Recombinant Human CAMK2A/CAMKA Protein (GST Tag)

• Background:

  Ca2+/calmodulin-dependent protein kinase2A (CAMK2A) belongs to the serine/threonine protein kinase family and, together with other 28 different isoforms, belongs to the Ca2+/ calmodulin-dependent protein kinase subfamily. CaM kinase II is thought to be an important mediator of learning and memory and is also necessary for Ca2+ homeostasis and reuptake in cardiomyocytes chloride transport in epithelia, positive T-cell selection, and CD8 T-cell activation. CAMKIIA is one of the major forms of CAMKII. It has been found to play a critical role in sustaining activation of CAMKII at the postsynaptic density. Studies have found that knockout mice without CAMKIIA demonstrate a low frequency of LTP. Additionally, these mice do not form persistent, stable place cells in the hippocampus.

• Synonyms:

  CAMKA

• UniProt:

  A8K161

• Accession Number:

  NP_741960.1

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-GST

• Sequence:

  Met 1-His 478

• Applications:

  Enzyme

• Field of Research:

  Signal Transduction; Cardiovascular; Neuroscience; Cancer; metabolism

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by reducing SDS-PAGE.

• Bioactivity:

  The specific activity was determined to be 160 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  80.3 kDa

• Observed Molecular Weight:

  80 kDa

• Formulation:

  Supplied as sterile solution of 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM Reduced Glutathione, pH 8.0

• Species:

  Human