Recombinant Human Interferon γ/IFNG Protein

• Background:

  IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF.

• Abbreviation:

  Interferon γ, IFNG

• Synonyms:

  IFN-Gamma; IFNG; Immune Interferon; Interferon Gamma

• UniProt:

  P01579

• Accession Number:

  P01579

• Expression System:

  HEK293 Cells

• Tag:

  None

• Sequence:

  Gln24-Gln166

• Field of Research:

  Cancer; immunology; Stem cells

• Endotoxin:

  < 1.0 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  16.8 kDa

• Observed Molecular Weight:

  16 & 20 - 25 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
  Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human