Total Bile Acid (TBA) Colorimetric Assay Kit
CAT:
763-E-BC-K181-M-03
Size:
48 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Total Bile Acid (TBA) Colorimetric Assay Kit
- Synonyms: TBA
- Field of Research: Liver and Renal Function
- Detection Method: Colorimetric method
- Assay Principle: With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.
- Assay Performance Time: 25 min
- Detection Range: 1.77-40 μmol/L
- Sensitivity: 1.36 μmol/L
- Shipping Conditions: Ice bag
- Storage Conditions: This product can be stored at 2~8°C for 12 months with shading light.