Total Carbohydrate Colorimetric Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Glycolysis and Lipid Metabolism; Plant Physiology Series

• Assay Principle:

  Carbohydrate is one of the important components of various bodies, and is also the main raw material and storage material of metabolism. Total carbohydrate mainly refers to reducing glucose, fructose, pentose, lactose, maltose, sucrose that can be hydrolyzed to reducing monosaccharides under determined conditions, and possibly partially hydrolyzed starch. The total carbohydrate was hydrolyzed to reducing sugar, which was reduced to amino compounds after co-heating with 3, 5-dinitrosalicylic acid under alkaline conditions. The amount of reducing sugar was proportional to the color of the reddish brown substance, and the total carbohydrate content in the sample was determined by this method.

• Assay Performance Time:

  40 min

• Sample Type:

  Serum, plasma; tissue

• Detection Range:

  0.036-1.0 mg/mL

• Sensitivity:

  0.036 mg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2-8°C for 12 months.

• Inter CV:

  2.1-5.3%

• Intra CV:

  1.0-2.9%

• Detection Instrument:

  Microplate reader