Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit

• Target:

  NEFA

• Type:

  Colorimetric method

• Field of Research:

  Glycolysis and Lipid Metabolism; Metabolic Diseases

• Assay Principle:

  Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated by measuring the OD value at 715 nm.

• Assay Performance Time:

  60 min

• Sample Type:

  Serum; animal tissue

• Detection Range:

  0.05-2.0 mmol/L

• Sensitivity:

  0.05 mmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S0092867422015379

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S2095809922007123

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S0048969722056844

• Citation 04:

  https://diabetesjournals.org/diabetes/article-abstract/71/9/2034/147087

• Citation 05:

  https://www.mdpi.com/1871912

• Citation 06:

  https://www.sciencedirect.com/science/article/pii/S2589004222009804

• Citation 07:

  https://www.frontiersin.org/articles/10.3389/fphar.2018.00345/full?report=reader

• Citation 08:

  https://www.sciencedirect.com/science/article/pii/S0014299921002314

• Citation 09:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7167271/

• Citation 10:

  https://www.sciencedirect.com/science/article/pii/S1382668920300636

• Citation 11:

  https://link.springer.com/article/10.1007/s00438-023-01993-8

• Citation 12:

  https://www.sciencedirect.com/science/article/pii/S0009898119318455

• Inter CV:

  6.1%

• Intra CV:

  2.3%

• Detection Instrument:

  Spectrophotometer