Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit

• Target:

  FFA; NEFA

• Type:

  Fluorometric method

• Field of Research:

  Metabolic Diseases; Glycolysis and Lipid Metabolism

• Assay Principle:

  Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.

• Assay Performance Time:

  70 min

• Sample Type:

  Serum; plasma; animal tissue; cells

• Detection Range:

  1.20-100 μmol/L

• Sensitivity:

  1.20 μmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://www.nature.com/articles/s41467-022-35481-1

• Citation 02:

  https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332

• Citation 03:

  https://link.springer.com/article/10.1186/s13098-022-00921-x

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/S0378874122009011

• Citation 05:

  https://www.sciencedirect.com/science/article/pii/S0955286319301330

• Citation 06:

  https://link.springer.com/article/10.1007/s11356-021-14871-w

• Citation 07:

  https://www.tandfonline.com/doi/abs/10.1080/01480545.2020.1784190

• Inter CV:

  5.1%

• Intra CV:

  4.1%

• Detection Instrument:

  Fluorescence Microplate Reader